Resultado da pesquisa (2)
Termo utilizado na pesquisa Garcia M.
#1 - Uso da ciclofosfamida em modelo experimental de imunodepressão experimental em ovinos
Abstract in English:
Garcia M., Sertório S. P., Alves G. J., Chate S. C., Carneiro S. & Lallo M.A. 2004. [Ovine experimental immunosuppression using cyclophosphamide.] Uso da ciclofosfamida em modelo experimental de imunodepressão experimental em ovinos. Pesquisa Veterinária Brasileira 24(3): 115-119. Curso de Medicina Veterinária, Universidade Paulista, Rua Enjolras Vampré 146, São Paulo, SP 04290-070, Brazil. E-mail: mgar@mgar.vet.br
Cyclophosphamide (CY) was used to evaluate the effect on the immune system of sheep. Castred adult rams were divided into 3 groups, with 6 animals each one. Group I (day 0) and Group II (day 1) were treated with CY (40 mg/kg, single dose, IV), and Group III was not treated and remained as control. All groups were immunized on day 0 with B19 brucellosis vaccine. On day 6, all animals were bled and serum agglutination test for brucellosis antibodies detection was performed. During 7 days blood lymphocyte counts and electrophoresis gammaglobulin dosage were daily performed. The results showed statistical decrease of immune response. Low serum titers of brucellosis antibodies were found in Groups I and II, and lymphopenia and hypogammaglobulinemia were also found in these groups. A high mortality rate (40%) occurred in the treated animals.
Abstract in Portuguese:
Garcia M., Sertório S. P., Alves G. J., Chate S. C., Carneiro S. & Lallo M.A. 2004. [Ovine experimental immunosuppression using cyclophosphamide.] Uso da ciclofosfamida em modelo experimental de imunodepressão experimental em ovinos. Pesquisa Veterinária Brasileira 24(3): 115-119. Curso de Medicina Veterinária, Universidade Paulista, Rua Enjolras Vampré 146, São Paulo, SP 04290-070, Brazil. E-mail: mgar@mgar.vet.br
Cyclophosphamide (CY) was used to evaluate the effect on the immune system of sheep. Castred adult rams were divided into 3 groups, with 6 animals each one. Group I (day 0) and Group II (day 1) were treated with CY (40 mg/kg, single dose, IV), and Group III was not treated and remained as control. All groups were immunized on day 0 with B19 brucellosis vaccine. On day 6, all animals were bled and serum agglutination test for brucellosis antibodies detection was performed. During 7 days blood lymphocyte counts and electrophoresis gammaglobulin dosage were daily performed. The results showed statistical decrease of immune response. Low serum titers of brucellosis antibodies were found in Groups I and II, and lymphopenia and hypogammaglobulinemia were also found in these groups. A high mortality rate (40%) occurred in the treated animals.
#2 - Leucogram evaluation of bovine holsteio females naturally infected by the Bovine Leukosis Virus
Abstract in English:
Lymphocytosis caused by Enzootic Bovine Leukosis (EBL) has been known since the beginning of the century. For the clinical diagnosis of EBL "hematologic keys" have been largely used. The objective of this paper was to evaluate the hematologic condition of black and white holstein cows infected by the Bovine Leukosis Virus (BLV) giving special attention to lymphocytic morphology. Blood samples from 423 cows reacting to the Agar Gel Immunodiffusion Test (AGID) and from 374 non reacting cows were collected. No statistical differences in polymorphic nuclear leucocyte counts and monocyte counts between reacting and non reacting cows were found, but the AGID-reacting cows showed higher lymphocyte counts than the non AGID-reacting cows. When the morphology of the lymphocytes was analysed it was found that the monocytic cell counts and the double nucleus lymphocyte counts were higer in AGID-reagent animals.
Abstract in Portuguese:
Desde o início do século já era conhecido que os animais afetados pela Leucose Enzoótica dos Bovinos (LEB) apresentavam linfocitose. Esta alteração foi muito usada como recurso auxiliar no diagnóstico clínico da LEB através do emprego das chamadas "chaves leucométricas". O presente estudo procurou avaliar o perfil do quadro leucocitário de fêmeas bovinas da raça holandesa naturalmente infectadas pelo Vírus da Leucose Bovina (VLB) dando-se particular atenção à morfologia dos linfócitos. Para tanto, realizou-se o leucograma de 423 bovinos reagentes e 374 não reagentes à prova de Imunodifusão em Gel para a Leucose Bovina (IDLB). Os resultados obtidos mostraram que apenas os linfócitos apresentaram aumentos estatisticamente significantes nos animais reagentes à IDLB. No tocante ao aspecto morfológico dos linfócitos, conclui-se que os linfócitos monocitóides e os linfócitos com núcleo duplo apresentaram contagens significativamente maiores nos animais reagentes.
